Strain comparisons in aquaculture species: a manual

When different strains or breeds of a particular species are available, the best choice is seldom immediately obvious for producers. Scientists are also interested in the relative performance of different strains because it provides a basis for recommendations to producers and it often stimulates the conduct of work aimed at unraveling the underlying biological mechanisms involved in the expression of such differences. Hence, strain or breed comparisons of some sort are frequently conducted.

Selective breeding in fish and conservation of genetic resources for aquaculture

To satisfy increasing demands for fish as food, progress must occur towards greater aquaculture productivity whilst retaining the wild and farmed genetic resources that underpin global fish production. We review the main selection methods that have been developed for genetic improvement in aquaculture, and discuss their virtues and shortcomings. Examples of the application of mass, cohort, within family, and combined between-family and within-family selection are given.

Risk analysis in aquaculture: A step-by-step introduction with worked examples

This publication is based on materials covered and outputs generated during the Workshop on Risk Assessment Methodologies and Tools for Aquaculture in Sub-Saharan Africa, which was jointly held by WorldFish and FAO in Siavonga, Zambia on 28 June - 2 July 2010. The workshop was delivered as a training exercise to 17 participants from seven sub-Saharan countries and was designed to highlight current methodologies and tools available for environmental risk analysis in aquaculture development.

PmPPAF is a pro-phenoloxidase activating factor involved in innate immunity response of the shrimp Penaeus monodon

One of the major steps in the innate immune response of shrimp includes the activation of serine proteinases of the pro-phenoloxidase pathway by the prophenoloxidase activation enzyme (PPAF). In this study, the cDNA encoding a serine proteinase homologue (SPH) with prophenoloxidase activating activity of Penaeus monodon (PmPPAF) was cloned and characterized.

Performance of the Genetically Improved Farmed Tilapia (GIFT) strain over ten generations of selection in Malaysia

A selection programme using Best Linear Unbiased Prediction (BLUP) method for the estimation of genetic merit was implemented by the Department of Fisheries Malaysia (DOF) in collaboration with the WorldFish Centre. This collaborative programme provided opportunities for further improvement of the GIFT strain in Malaysia. The overall aim of the present study was to evaluate the performance of GIFT strain during the long-term selection programme in Malaysia.

Individual growth monitoring of European sea bass larvae by image analysis and microsatellite genotyping

The aims of the present study were to develop non-lethal methods to identify individual fish larvae and post-larvae before tagging and accurately follow their growth characteristics. European sea bass (Dicentrarchus labrax) was used as a model species at four different ages ranging from 71 to 100 days post fertilization (dpf).

Genotype by production environment interaction in the GIFT strain of Nile tilapia (Oreochromis niloticus)

Three discrete generations of GIFT fish (Nile tilapia strain, Oreochromis niloticus; a total of 10,065 fish with pedigree and phenotypic information) were tested in pond and cage culture environments to determine genotype by production environment interaction between both environments in Malaysia. Live weight (selected trait), standard length, body depth and width were recorded. A bivariate animal model was used to estimate variance and covariance components, whereby the homologous body traits in pond and cage environments were treated as genetically distinct traits.

Genetically Improved Farmed Tilapia (GIFT)

Genetic improvement through selective breeding has been used for millennia on crops and livestock, but up until the 1980s, little had been done to utilize this process for farmed fish. In response to the inadequate supply of tilapia seed and the deteriorating performance of the fish in many aquaculture systems in Asia, WorldFish and partners began the Genetic Improvement of Farmed Tilapia (GIFT) project to develop a faster-growing strain of Nile tilapia (Oreochromis niloticus) that was suitable for both small-scale and commercial aquaculture.

Genetic improvement of farmed tilapias: Genetic parameters for body weight at harvest in Nile tilapia (Oreochromis niloticus) during five generations of testing in multiple environments

The main objective of this paper was to report more reliable estimates of the genetic variation and the genotype by test environment interaction for harvest body weight in the GIFT population in the Philippines than could be obtained from the base population by using the data from the five generations following the base population and that covers a wider span of test environments than the later experiments referred to above. Included are also estimates of the genotype by sex interactions for body weight.

Genetic and non-genetic indirect effects for harvest weight in the GIFT strain of Nile tilapia (Oreochromis niloticus)

The main objective of this study was to quantify the heritable variation for growth rate of GIFT, and the contribution of IGEs to this heritable variation. Here we describe the experiment conducted for this purpose, and present estimated parameters of genetic and non-genetic indirect effects on growth rate in the GIFT strain.

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