A growth trial was conducted to investigate the effect of different dietary protein and feeding times on growth performance and feed utilization of freshwater prawn Macrobrachium rosenbergii Post-Larvae (PL). The experiment was conducted in outdoor concrete pens (6 m-3) for 84 days. Post-larvae with an average weight of 20.8±0.17 mg were stocking at 30 PL m-3 in each pens. Two isocaloric (~15.0 MJ DE kg-1) test diets were formulated to contain two different dietary protein 35 and 40%. The daily feeding level was divided into equal two amounts and fed twice a day at three different feeding times (9:00; 12:00 h); (9:00; 15:00 h) and (9:00; 18:00 h). The highest survival rate, weight gain and specific growth rate (p