Learn.ink is an online platform for digitized learning and training. Sharing digital content on mobile devices is important for efficient knowledge sharing and training to work with target community groups on a range of topics, such as fish epidemiological surveys, disease surveillance, fish sampling for disease diagnostics and better management practices for biosecurity. Using the Learn.ink platform, we aim to provide a more engaging digital learning experience for end users in situations where remote virtual training is necessary. The lessons and quizzes for these introductory fish sampling microcourses were developed on Learn.ink. The lessons aim to provide end users with step-by-step guidance to learn the necessary skills to collect biological samples for different diagnostic techniques, including wet mount preparation, microbiome, blood, bacteriology, molecular biology, virology and histology. The quizzes aim to test the knowledge of end users after completing the lessons. End users include but are not limited to field enumerators, post-graduate students, extension workers, service providers and lead farmers. After completing the fish sampling course, end users can put into practice the fish sampling techniques they learned for (1) a disease outbreak investigation, (2) follow-up routine sampling visits among catfish and tilapia farmers, (3) a research project, or (4) part of a regional or national disease surveillance program. These guidelines provide the links for end users to access the fish sampling microlearning curriculum on Learn.ink. The microlearning curriculum consists of introduction and foundation microcourses that should be completed by end users before taking any of the microcourses on the six sampling protocols, that cover sampling for wet mount, microbiome, blood, bacteriology, molecular diagnostics and virology, as well as histology.