This manual provides a comprehensive step-by-step guide to running a tilapia hatchery operation. It includes advice on site selection, site preparation, hatchery design and construction, farming practices (broodstock management, spawning, sex reversal, hormonal feed preparation and feeding), fingerling harvesting and marketing, biosecurity and fish health management, good farm management practices and recordkeeping – all of which are critical elements of a successful tilapia hatchery operation.