The CGIAR is a global research partnership for a food-secure future. It works through 15 global research centres located worldwide. WorldFish is part of One CGIAR, the world’s largest agricultural innovation network. WorldFish is the only center in One CGIAR with 45 years of experience in fisheries and aquaculture research in low-and middle-income countries. ICAR, as the nodal body, has signed Memorandum of understanding or Agreement with 12 CGIAR Centres. Indian Council of Agricultural Research (ICAR) and WorldFish have signed a MOU in July 1996. Under the MOU and subsequent MOAs, ICAR is providing funding support under W3 to establish long term research collaboration between the two organizations in the areas of sustainable aquaculture (SA), small scale fisheries (SSF) and value chains and nutrition (VCN). This collaboration seeks alignment of research priorities of three major ICAR research institutions, namely Central Institute of Freshwater Aquaculture (CIFA) with SA flagship, Central Inland Fisheries Research Institute (CIFRI) with SSF Flagship and Central Institute for Fisheries Technology (CIFT) with VCN flagship of WorldFish. Agricultural research and education in India is precisely undertaken in the areas of mutual interest identified in the Work Plans, which are developed for five years. An annual meeting of the Heads of CGIAR centres in India is held with the senior officers of the ICAR in which all the ongoing programmes are discussed and priorities are decided considering the national requirements and the areas where the CGIAR centres could assist the ICAR institutions and agricultural universities to address the existing as well as the emerging issues. The Annual Report 2021 provides a summary of salient outputs and outcomes of the joint R&D activities of WorldFish with 3 premier ICAR Fisheries Institutions namely CIFA, CIFRI and CIFT.