Twelve enzymes were assayed in Clarias gariepinus collected from India and Thailand. Genetic variation was observed at eight loci, viz EST-1* and 2*, AAT*, ODH*, MDH* GPI* L, GPI-1*M and HK*. Observed heterozygosities were found to be 0.149 and 0.152 , and percent polymorphism 50% and 31.25% respectively in samples from India and Thailand. The results indicate that C. gariepinus in India could be the mix of different stocks belonging to diferrent genetic lineages.