Despite the success in fertilization and hatching of fish eggs with cryopreserved sperm, report on growth and survival of larvae produced from frozen-thawed sperm is inadequate. This study evaluates the applicability of cryopreserved sperm for mass seed production by comparing the growth and survival of a popular food-fish olive barb, Puntius sarana (Hamilton 1822) larvae produced from cryopreserved and fresh sperm.