Ecosystem-based management of fisheries and other transboundary natural resources require a number of organizations across jurisdictions to exchange knowledge, coordinate policy goals and engage in collaborative activities. Trust, as part of social capital, is considered a key mechanism facilitating the coordination of such inter-organizational policy networks. However, our understanding of multi-dimensional trust as a theoretical construct and an operational variable in environmental and natural resource management has remained largely untested. This paper presents an empirical assessment of trust and communication measures applied to the North American Great Lakes fisheries policy network.