Aquaculture industry in Bangladesh is significantly contributing profound positive aspects to the socioeconomic structures of the country. Irrational and inappropriate use of antimicrobials in aquaculture and associated transfer risk of antimicrobial resistant pathogens from aquaculture settings through interlinked human-animal-environment spread pathway may affect enhanced sustainability of the sector, a One Health problem. In this study, prevalence and antimicrobial resistance in one of the key One Health significance pathogens, Escherichia coli from farmed aquatic foods were investigated.