This practical manual provides a unique, step-by-step guideline for induced breeding of Punti - pool barb (Puntius sophore) and swamp barb (Puntius chola) to produce mass-scale seed in a hatchery. The breeding protocol is straightforward and easy for existing fish hatchery operators to adopt. This nine-step guideline serves as a comprehensive protocol that any fish hatchery interested in breeding punti can follow, making a significant contribution to the development of nutrition-sensitive aquaculture.