Fish is the main animal-source food consumed, accounting for 60% of animal protein intake and is the most frequently consumed nutrient rich food in Bangladesh Pangasius is now the most consumed fish in rural areas of Bangladesh. Pangasius aquaculture has improved access to fish among poor consumers in Bangladesh by increasing supply and reducing relative prices. The present study assesses the production costs, profitability and the importance of inputs (seed, feed, fertilizer and labor) in striped catfish (Pangasianodon hypophthalmus) production under polyculture system in Bangladesh.