Antimicrobial resistance (AMR) is a globally recognized One Health problem posing major threats on public health management of infectious diseases. Aquaculture is one of the leading food sectors that is challenged by infectious diseases leading to irrational and inappropriate use of antimicrobials. Antimicrobial resistant pathogens from aquaculture can find their way to terrestrial animals, human and their environments, and vice versa, thereby affecting the whole ecosystem. We collected 132 fish/shrimp samples from two wet markets for to generate evidence of antimicrobial resistance in key pathogens from farmed aquatic foods and surveyed 672 aquaculture farms to characterize antimicrobial use (AMU) in commercial aquaculture in Bangladesh.